• get familiar with the figure below ASAP
  • ci=λ repressor
  • PL PR : lytic (strong promoter)
  • PRM: lysogenic (weak promoter) PRE:weak
  • 3 operons from right to left (overlapping with two promoters: PRM & PR)
    • OR1: ci (lambda repressor binding site)
    • OR2:cro binding site
    • OR3:for both, lower affinity
  • positive feedback: cro/ci binds with the operon on the opposite site respectively
  • Cro is a repressor, but lambda repressor (ci) is not only a repressor
  • N:anti-termination
  • ci
    • 四聚体
  • PRE(repression establish):
    • weak promoter
    • makes antisense cro
  • cII: orc